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comment by iammyownrushmore

Scale it to what? Like, use this process with more neurally complex organisms? Implementing this was rate limited by the available technology, but mapping that motherfucker? mechanosm on my comment pointed me to the protocol (granted that was in 1986) but, while automation is our friend nowadays, it's not any less tedious. My experience is limited to mapping neurons using confocal in 50 micron sections, but that was not terribly efficient.

Just physically linking the neurons is bitch in and of itself, but then you have to confirm each neuron type, response, etc. It's not as simple as "draw the roads on the map, and now you drive across the country." bad metaphor but whatever.