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thundara  ·  3274 days ago  ·  link  ·    ·  parent  ·  post: Pubski: March 9, 2016

It's exciting for now, but I'm sure in 6 months it will be a chore. But hey, it opens up the doors past "wait 6 months for our next batch of mice".

    What cell line are you using? Or is it a primary culture?

BV-2 and N9, both "microglia". Primary culture may follow later, but they give a somewhat limited amount of material (in exchange for being /actual/ microglia), so I'm getting familiar with assays and testing inhibitors in these guys first. Plus my lab isn't equipped to do primary or iPSC-derived cells, so I have to walk across the street to collaborator's lab for that.

    Also what is that thing jesus it looks huge.

See the rather-large table-top instrument on the left. We've got a way more ghetto HPLC (right) setup than what's pictured there though.