I always have the feeling the proteomic methods (specially SDS-PAGE) are the least advanced compared to all the DNA/RNA stuff. Of course, this has a lot to do with the instability of proteins and handling but still, it is annoying.

Most certainly. Until you get true peptide sequencing (a la nanopore?) / some method of amplification, there will be huge sensitivity limits to proteomics that will never bring it to the single-cell level. Supposedly new multiplexed staining methods should help with that, but antibody-based readouts have their own set of issues... I'm hopeful for advances in that area next few years though.

The next batch of suitable mice is in 5 weeks? fuck this shit.

The mice I've been using take close to four months, plus however many days of gene induction, plus the wait time for this breed, which has been quite a while now. Definitely makes me nervous in picking which assays I run. I've been stuck on whether to throw my remaining sample for a kinase-activation assay, or to save it for more targeted blots...

Bouldering is great though! After the first few rounds of forearm death, progress happens pretty quickly. I've been going since September and seen a grade increase ~every two months.