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comment by thundara
thundara  ·  236 days ago  ·  link  ·    ·  parent  ·  post: We made a video for Forever Labs -- I dig it.

TREMENDOUS.

But seriously, I'm curious, how many cells do you end up getting from each patient and what fraction of those actual survive the freeze / thaw procedure?




mk  ·  235 days ago  ·  link  ·  

Roughly 20M MNCs/ml but there is variability. In terms of CFUs, we haven't discerned a difference between the same sample when comparing a frozen aliquot to a fresh one.

We can greatly adversely affect the recovery modifying our culture conditions, however.

elizabeth  ·  235 days ago  ·  link  ·  

I was wondering - is there a way to multiply those cells in the future? I just imagine i'd be pretty sad if the cells run out, exactly when i would need them the most

mk  ·  235 days ago  ·  link  ·  

Yes, there is, and that is the plan. You can expand what we collect to exceed the number of cells in your own body. It's my plan to expand both my blood/immune-building hematopoietic stem cells and my bone/connective tissue-building mesenchymal stem cells to ridiculous numbers, and then to freeze those in many individual chambers.

As it stands, we store your cells in 3 large chambers, and 4-5 small ones, so that you can draw up a fraction of them without thawing the rest.

elizabeth  ·  235 days ago  ·  link  ·  

Awesome! Looks like you thought of everything :)

thenewgreen  ·  235 days ago  ·  link  ·  

I'm actually about to head in to a procedure. b_b, can you reply?