From the early 1980s until his death in 1996, Packard dedicated much of his time and money to philanthropic projects. Prompted by his daughters Nancy Packard Burnett and Julie Packard, in 1978 Dave and Lucile Packard created the Monterey Bay Aquarium Foundation. The couple eventually donated $55 million to build the new aquarium, which opened in 1984 with Julie Packard as executive director. In 1987, Packard gave $13 million to create the Monterey Bay Aquarium Research Institute, and the David and Lucile Packard Foundation has since provided about 90% of the Institute's operating budget.

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