A month and a half of treating, sorting, maintaining, and cleaning my cells every day to finally get the CRISPR knockouts I'd been working towards:
Now to do this 6 more times.
One of the genes I'd been trying to knock out was in a tricky region to sequence. I asked my adviser if I should spend a month debugging the PCR (after a post-doc had tried and failed for a month), just show the protein had disappeared by an antibody assay, or sequence the whole genome and look at that one gene afterwards.
They said sequence the whole genome.
The golden days we live in. Time to start making designer babies.